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There is science in this... katG gene...its just naturally insensitive
Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis.
B Heym, Y Zhang, S Poulet, D Young and S T Cole
Unit de Gntique Molculaire Bactrienne, Institut Pasteur, Paris, France.
ABSTRACT
The isoniazid susceptibility of Mycobacterium tuberculosis is mediated by the product of the katG gene which encodes the heme-containing enzyme catalase-peroxidase. In this study, the chromosomal location of katG has been established and its nucleotide sequence has been determined so that the primary structure of catalase-peroxidase could be predicted. The M. tuberculosis enzyme is an 80,000-dalton protein containing several motifs characteristic of peroxidases and shows strong similarity to other bacterial catalase-peroxidases. Expression of the katG gene in M. tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid....
BEAUTIFUL....So in conclusion...my hypothesis is supported.. ...i.e what that dude said....KATG gene....*see above....
Done........... and dont ask ....some things are beter off unknown....
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